鸭I-FABP基因的克隆、组织表达模式及功能研究

doi:10.11843/j.issn.0366-6964.2016.05.023

畜牧兽医学报 ›› 2016, Vol. 47 ›› Issue (5): 1041-1048.doi: 10.11843/j.issn.0366-6964.2016.05.023

鸭I-FABP基因的克隆、组织表达模式及功能研究

陈芳^1，2，3*，张昊^1，2，3，卢立志³

（1.湖北省农业科学院畜牧兽医研究所，武汉 430064； 2.动物胚胎工程及分子育种湖北省重点实验室，武汉 430064；3.浙江省农业科学院畜牧兽医研究所，杭州 310021）

收稿日期:2015-06-25 出版日期:2016-05-23 发布日期:2016-05-23
通讯作者: 陈芳，E-mail:zhhchenfang0730@hotmail.com
作者简介:陈芳（1987-），女，四川眉山人，助理研究员，博士，主要从事动物营养调控机理研究
基金资助:
湖北省创新团队项目(2016-620-000-001-028)；动物胚胎及分子育种湖北省重点实验室开放课题及湖北省农业科学院青年基金（2015NKYJJ27）

Molecular Cloning，Expression Pattern and Function of Duck I-FABP Gene

CHEN Fang^1，2，3* ，ZHANG Hao^1，2，3，LU Li-zhi³

（1.Institute of Animal Husbandry and Veterinary Sciences，Hubei Academy of Agricultural Sciences，Wuhan 430064，China；2.Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding，Wuhan 430064，China；3.Institute of Animal Husbandry and Veterinary Sciences，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China)

Received:2015-06-25 Online:2016-05-23 Published:2016-05-23

摘要/Abstract

摘要：

本研究旨在探讨鸭I-FABP基因的结构与功能。本试验以绍兴麻鸭为研究材料，运用RACE、RT-PCR 方法扩增鸭I-FABP基因mRNA全序列，运用qRT-PCR方法检测该基因在小肠不同区段的表达特性，运用siRNA干扰、qRT-PCR分析其功能。结果表明，鸭I-FABP基因共编码132个氨基酸，在不同物种间保守性较高，且在第60位氨基酸处发现一个Ile/Thr的突变位点；I-FABP在空肠前半段和十二指肠中表达量较高，与微粒体甘油三酯转移蛋白 (Microsomal triglyceride transferprotein，MTTP)和载脂蛋白B（Apolipoprotein B，ApoB）基因在小肠不同肠段具有相似的表达变化情况，而与肝脏型脂肪酸结合蛋白基因(Liver fatty acid binding protein，L-FABP)存在差异；干扰I-FABP基因表达后，MTTP和ApoB基因的表达量显著降低，而L-FABP未发生显著变化。该结果预示了I-FABP基因在鸭的小肠脂肪酸吸收与转运通路中的重要作用，也为该基因的深入研究奠定了基础。

Abstract:

This study aimed to analyze the structure and function of duck I-FABP gene.The Shaoxing duck was used.The sequence of duck I-FABP mRNA was cloned by RACE and RT-PCR.The expression patterns of I-FABP in different parts of small intestine were detected by qRT-PCR.The function was analyzed by siRNA and qRT-PCR.The duck I-FABP gene encodes a 132-amino acid protein.A mutation from T to C resulting in a substitution from Ile to Thr was found at codon 60 in exon 2 of the I-FABP.I-FABP，MTTP and ApoB mRNA had high expression levels in duodenum and the first half of jejunum and had slight differences with the expression of L-FABP.After the I-FABP interfered，the expression of MTTP and ApoB were decreased significantly，but no obvious change at the expression level of L-FABP.The results verify the important role of I-FABP in uptake and translocation of fatty acid in small intestine of ducks and provide the foundation for further research.

中图分类号:

S834
S813.3

陈芳，张昊，卢立志. 鸭I-FABP基因的克隆、组织表达模式及功能研究[J]. 畜牧兽医学报, 2016, 47(5): 1041-1048.

CHEN Fang，ZHANG Hao，LU Li-zhi. Molecular Cloning，Expression Pattern and Function of Duck I-FABP Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(5): 1041-1048.

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鸭I-FABP基因的克隆、组织表达模式及功能研究

Molecular Cloning，Expression Pattern and Function of Duck I-FABP Gene

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